鞍山师范学院学报

1-萘乙酸常被用于提高农产品的产量，但其过量残留对人体的危害较大。基于表面增强拉曼光谱技术，建立了一种快速检测番茄中1-萘乙酸残留的方法。以银纳米粒子溶胶为活性基底，以NaCl为聚沉剂，考察混合顺序、银纳米粒子溶胶的用量、NaCl溶液的用量、待测物的用量、混合时间等因素对拉曼光谱强度的影响。选择833 cm^-1处散射峰作为1-萘乙酸拉曼光谱的特征峰，将100μL浓度为1 mol·L^-1的NaCl溶液、150μL样品溶液、450μL C-AgNPs溶液依次混合均匀，用超纯水定容至1 mL，在室温下静置12 min后进行测定，1-萘乙酸在1.0～2.0 mg·L^-1浓度范围内线性关系良好。该方法快速、简便，为番茄中植物生长调节剂检测提供了新思路。

1-Naphthalene acetic acid is commonly used to increase the yield of agricultural products,but their excessive residues pose significant risks to human health.Based on surface-enhanced Raman spectroscopy,a rapid method was established for detecting the residue of the plant growth regulator 1-Naphthalene acetic acid in tomatoes.Using silver colloid as the active substrate and NaCl as the aggregating agent,the effects of various factors on the SERS intensity were investigated,including the mixing order of the system,the amount of silver nanoparticle colloid,the amount of NaCl solution,the amount of analyte,and the mixing time.The characteristic peak of 1-Naphthalene acetic acid at 833 cm^-1 was selected as the marker for Raman spectroscopy.The optimized experimental procedure was established as follows:100 μL of a 1 mol·L^-1 NaCl solution,150 μL of the sample solution,and 450 μL of the C-AgNPs solution were thoroughly mixed.The mixture was then diluted to a final volume of 1.0 mL with ultrapure water,incubated at room temperature for 12 min,and subsequently subjected to measurement.A good linear relationship was observed for 1-Naphthalene acetic acid in the concentration range of 1.0～2.0 mg·L^-1,demonstrating its applicability for sample detection.This method is rapid and simple,providing a new idea for the detection of plant growth regulators in tomatoes.