鞍山师范学院学报

优化了超声辅助提取黄柏提取物的工艺条件，并测定了黄柏提取物对多种真菌的抑菌活性。采用单因素试验结合正交试验，优化提取的料液比、时间、温度及次数；通过固体琼脂斜面法和泡沫塑料柱法测定提取物对10种真菌的抑菌活性，并与对照药1#、对照药2#进行对比。实验结果表明，最佳提取条件为料液比1∶30、提取时间40 min、提取温度40℃、提取次数4次；黄柏提取物对敏感菌株的最低抑菌浓度为0.125～1.00 mg/mL，显著低于对照药1#(1.00～10.00 mg/mL)；黄柏提取物和中药对照药1#对不敏感菌株（木霉菌和毛霉菌）的最低抑菌浓度一致。黄柏提取物具有优良的抑真菌活性，其抑真菌效果显著强于对照药1#，对部分真菌的抑制作用优于对照药2#。

The process conditions for ultrasound-assisted extraction of Phellodendron amurense extract were optimized,and its antibacterial activity against various fungi was determined Single-factor experiments combined with orthogonal experiments were employed to optimize the liquid-to-solid ratio,extraction time,temperature,and number of extractions.The antibacterial activity of extract against 10 types of fungi was determined using the solid agar slant method and the polystyrene column method,and compared with control drug 1# and control drug 2#.The experimental results showed that the optimal conditions were:a liquid-to-solid ratio of 1∶30,an extraction time of 40 min,an extraction temperature of 40 ℃,4 extractions.The minimum inhibitory concentration of Phellodendron amurense extract against sensitive strains was 0.125～1.00 mg/mL,which was significantly lower than that of the traditional Chinese medicine control drug 1#(1.00～10.00 mg/mL). The minimum inhibitory concentration Phellodendron amurense extract and the traditional Chinese medicine control drug 1# against insensitive strains(Trichoderma and Mucor) were consistent.Phellodendron amurense exhibits excellent antifungal activity,and its antifungal effect is significantly stronger than that of the traditional Chinese medicine control drug 1#,with its inhibitory effect on some fungi being superior to that of miconazole-containing chemical antifungal drug.