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采用超声波法从茉莉花中提取黄酮类化合物并得出最佳提取条件,物料比为1∶25,80%乙醇做溶剂,超声提取时间为50 min时黄酮提取率较高.用紫外光谱法测定黄酮化合物的含量,芦丁标准溶液线性回归方程为A=0.956 1C-0.005 1,相关系数r=0.999 0,此方法的回收率为98%~100%,变异系数0.015%,方法快速,准确度与精密度均较高.
Abstract：The ultrasonic extraction method is used for extracting flavonoids in Jasmine,the optimum extracting conditionswere investigated. The optimum conditions of ethanol-extracting are obtained as follows:80%ethanol,the ratio of material to solvent is 1∶ 25,the time is 50 min. The flavonoids in jasmine are determined by sepctrophotometry. A standard curve is A = 0. 956 1C- 0. 005 1,The results show that the recovery is 98% ~ 100% and the coefficient of variation is0. 015%,the accuracy and precision of method is high.
[1]黄桂丽.茉莉的化学成分与药理作用的研究进展[J].中外医疗.2010,11(33):113-114.
[2]李世敏.功能食品加工技术[M].北京:中国轻工业出版社,2003,121.
[3]杨淑文,马兴,汪业菊.柚皮总黄酮的提取及其抗氧化活性研究[J].辽宁师范大学学报:自然科学版,2011,34(2):225.
[4]Harbome J B.The Flavonoids Advances in Research since 1986[M].London:Chapman&Hall,1994,676.
[5]Yu O,McGonigle B.Metabolic engineering of isoflavone biosynthesis[J].Advances in Agronomy,2005,86:147.
[6]Habtemariam S.Flavonoids as inhibitors or enhancers of the cytotoxicity of tumor Necrisis factwra in L-929 tumor cells[J].J Nat Prod,1997,60(8):775.
[7]Hu C Q,Chen K,Shi Q,etal.Anti-AID sagents,10,acacetin-7-O-β-D-galactopyranoside,an anti-HIV principle from chrysanthemum 69 morifolivm and a structure activity correlation with some related flavonoids[J].J Nat Prod,1994,57(1):42.
[8]李凌智.新疆紫茉莉黄酮类化合物及色素的初步研究[D].新疆:新疆大学食品科学,2010.
[9]侯冬岩,回瑞华,李学成,等.洋葱皮中黄酮化合物的超声提取与光谱分析[J].食品科学,2006,27(9):172-174.
[10]罗建华.茉莉花植物总黄酮的超声波提取及鉴别[J].微量元素与健康研究,2007,24(5):49-51.
[11]袁建梅.合欢花中总黄酮的提取工艺及对羟自由基清除作用的研究[J].中国食品添加剂,2012,11(1):87-92.
[12]侯冬岩,回瑞华,刘晓媛.鸡冠花花叶茎中黄酮类化合物提取工艺的研究[J].鞍山师范学院学报,2008,10(2):11-14.
[13]回瑞华,杨梅,唐蕊.金银花中总黄酮的光谱分析及抗氧化性能测定[J].分析试验室,2004,23(11):52-56.
[14]宁坚刚.蒲公英花中黄酮类物质的提取和含量测定[J].青海大学学报,2004,22(4):59-61.
[15]石玉平.沙枣花中黄酮类化合物的提取[J].南京林业大学学报,2005,29(2):106-109.
[16]侯冬岩,回瑞华,许冰.佩兰中黄酮类化合物提取工艺的研究[J].鞍山师范学院学报,2006,8(6):14-18.
[17]侯冬岩,回瑞华,刘晓媛.万寿菊花叶茎中黄酮的含量及抗氧化性能的分析[J].鞍山师范学院学报,2008,10(4):15-18.
基本信息:
中图分类号:TQ461
引用信息:
[1]杨莉莉,侯冬岩,回瑞华.茉莉花中黄酮化合物的超声提取与测定[J].鞍山师范学院学报,2014,16(04):20-24.
2014-08-20
2014-08-20